Iodine SPOT

The measurement of urinary iodine (IU) provides an accurate approximation of dietary iodine intake. The majority of iodine that is ingested (90%) is excreted via the urine. When there is adequate amounts of iodine in the body to support various bodily systems, the body offsets excess by excreting it in the urine.  UI measurements provide a biological indicator of Iodine deficiency disorders by determining whether the body has sufficient levels.

Iodine's main action is involved in thyroid function. Due to the numerous actions carried out by the thyroid, the implications of iodine deficiency are significant.  The major hormone secreted by the thyroid is thyroxine, also called T4 because it contains 4 iodine atoms. To exert its effects, T4 is converted to T3 by the removal of one iodine atom. This occurs mainly in the liver and other tissues where T3 acts, such as the brain.  Other actions include the formation and integrity of normal breast tissue, foetal brain development and function, and anti-microbial effects.

Test Kit

After the practitioner has provided a request form, the patient can order their Iodine Spot test kit online at

Specimen Requirements

Urine Sample - Home Testing Kit

The test kits provided contain everything required to complete the test.

Patient Preparation

  • Avoid all sources of iodine for 48 hours prior to completing the test
    - Iodine is found in the following: iodised table salt, kelp, nori and other seaweeds, seafood, and Betadine®.***
  • Fast overnight from 10:00pm the evening before the morning urine specimen is collected (water may be consumed in this time)
  • Do not collect urine during menstruation

***Note: many multivitamin and mineral supplements contain iodine (check labels carefully).

Turnaround Time

The standard turnaround time for this test is 10 – 14 business days.



Additional $20.00 postage & handling fee applies to this test.


Prepayment is required when ordering test kit.



Companion Tests:

  • Thyroid Hormone Profile
  • Baseline Hormone Profile
  • Adrenal Hormone Profile

The results of the urinary iodine test may support or be supported by additional BeFunctional testing. Given the importance of Iodine for thyroid function, combining Urinary Iodine with the Thyroid Hormone Profile is important to ensure a comprehensive assessment of thyroid function. An underactive thyroid is also common in menopausal and post-menopausal women.

A Baseline Hormone Profile may be required to provide additional information in terms of identifying hormonal imbalance in menopausal and post-menopausal women.

The Adrenal Hormone Profile, which measure Cortisol and DHEA-S over a 24-hour period, may also be a useful test to consider as physical, mental and environmental stressors can inhibit the conversion of T4 to T3, this decreasing the amount of active thyroid hormone available to the cells.